Review



kb dna ladder  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Thermo Fisher kb dna ladder
    Kb Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+dna+ladder/DNA+Molecular+Weight+Marker%2C+1Kb+Ladder/pm42347511-158-3-6
    Average 97 stars, based on 1 article reviews
    kb dna ladder - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Comparison:

    Article Title: Microbial biodiversity in Tunisian olive grove soils: a reservoir of phytopathogenic fungi and potential beneficial microorganisms
    Article Snippet: Genomic DNA was extracted and purified from powdered lyophilized mycelia (each sample, 10–15 mg) using the Wizard Magnetic DNA Purification System for Food kit (Promega, Madison, WI, USA), according to the manufacture’s protocol. .. The quantity and integrity of DNA were checked by comparison with a standard 1 kb DNA Ladder (Fermentas GmbH, St. Leon-Rot, Germany) on 0.8% agarose gel, after electrophoretic separation. ..

    Article Title: In Vitro regeneration of bio-immunized banana cv. Grand Naine using a novel double-decker temporary immersion bioreactor
    Article Snippet: .. Bands were visualized and documented using a gel documentation system (Syngene), and amplicon sizes were estimated by comparison with a 1 kb DNA ladder (Fermentas) . ..

    Article Title: In Vitro regeneration of bio-immunized banana cv. Grand Naine using a novel double-decker temporary immersion bioreactor.
    Article Snippet: .. Bands were visualized and documented using a gel documentation system (Syngene), and amplicon sizes were estimated by comparison with a 1 kb DNA ladder (Fermentas) (Kadam et al., 2018). ..

    Agarose Gel Electrophoresis:

    Article Title: Microbial biodiversity in Tunisian olive grove soils: a reservoir of phytopathogenic fungi and potential beneficial microorganisms
    Article Snippet: Genomic DNA was extracted and purified from powdered lyophilized mycelia (each sample, 10–15 mg) using the Wizard Magnetic DNA Purification System for Food kit (Promega, Madison, WI, USA), according to the manufacture’s protocol. .. The quantity and integrity of DNA were checked by comparison with a standard 1 kb DNA Ladder (Fermentas GmbH, St. Leon-Rot, Germany) on 0.8% agarose gel, after electrophoretic separation. ..

    Article Title: Genetic diversity and gut microbiome of Anopheles mosquitoes in Tamil Nadu by using COI DNA barcoding and 16S rRNA metagenomics.
    Article Snippet: .. To confirm the PCR reactions, we used a 1.5% agarose gel containing ethidium bromide (EtBr) in 1X TAE buffer alongside a 100 kb DNA ladder (Thermo Fisher Scientific) and positive and negative controls. ..

    Staining:

    Article Title: Longitudinal genomic insights into resistance and virulence dynamics of Klebsiella pneumoniae clinical isolates from Pakistan.
    Article Snippet: Genomic DNA was extracted with the GeneJET Genomic DNA Purification Kit (catalog number K0722; Thermo ScientificTM, Waltham, MA, USA) and checked by running a gel electrophoresis with 1% agarose. .. The DNA was stained with ethidium bromide (10 mg/mL) and compared against a 1 kb DNA ladder (catalog number SM0312; Thermo ScientificTM, Waltham, MA, USA) for size reference. ..

    Article Title: Recombinant Human Fab Antibodies Differentially Neutralize Shiga Toxin in Renal Epithelial and Endothelial Cells.
    Article Snippet: .. Lane 1, 1 kb DNA ladder (Invitrogen); lane 2, anti-Stx1 Fab clone B6; lane 3, anti-Stx1 Fab clone C8; Figure S2: SDS–PAGE analysis (12%) of purified Fabs stained by silver nitrate. ..

    Polymerase Chain Reaction:

    Article Title: Genetic diversity and gut microbiome of Anopheles mosquitoes in Tamil Nadu by using COI DNA barcoding and 16S rRNA metagenomics.
    Article Snippet: .. To confirm the PCR reactions, we used a 1.5% agarose gel containing ethidium bromide (EtBr) in 1X TAE buffer alongside a 100 kb DNA ladder (Thermo Fisher Scientific) and positive and negative controls. ..

    Article Title: Molecular dissection of the UvrC protein involved in nucleotide excision repair in Listeria monocytogenes.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Purification:

    Article Title: Recombinant Human Fab Antibodies Differentially Neutralize Shiga Toxin in Renal Epithelial and Endothelial Cells.
    Article Snippet: .. Lane 1, 1 kb DNA ladder (Invitrogen); lane 2, anti-Stx1 Fab clone B6; lane 3, anti-Stx1 Fab clone C8; Figure S2: SDS–PAGE analysis (12%) of purified Fabs stained by silver nitrate. ..

    Article Title: Molecular dissection of the UvrC protein involved in nucleotide excision repair in Listeria monocytogenes.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Amplification:

    Article Title: In Vitro regeneration of bio-immunized banana cv. Grand Naine using a novel double-decker temporary immersion bioreactor
    Article Snippet: .. Bands were visualized and documented using a gel documentation system (Syngene), and amplicon sizes were estimated by comparison with a 1 kb DNA ladder (Fermentas) . ..

    Article Title: In Vitro regeneration of bio-immunized banana cv. Grand Naine using a novel double-decker temporary immersion bioreactor.
    Article Snippet: .. Bands were visualized and documented using a gel documentation system (Syngene), and amplicon sizes were estimated by comparison with a 1 kb DNA ladder (Fermentas) (Kadam et al., 2018). ..

    Plasmid Preparation:

    Article Title: Molecular dissection of the UvrC protein involved in nucleotide excision repair in Listeria monocytogenes.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Molecular Weight:

    Article Title: Molecular dissection of the UvrC protein involved in nucleotide excision repair in Listeria monocytogenes.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



    Similar Products

    96
    TaKaRa 1 kb dna ladder
    Validation of recombinant plasmids by restriction enzyme analysis and functional selection screening. (A) Restriction enzyme digestion of intermediate recombinant plasmids was performed using XhoI, XbaI , and KpnI . The observed banding patterns were consistent with the predictions generated by SnapGene software. Lanes 1–3 show the predicted digestion patterns of p15A-CmR-D90, p15A-CmR-D90-Δ5a/AmpR-ccdB, and p15A-CmR-D90-Δ5a/EGFP. Lanes 4–5, 6–7, and 8–9 correspond to the experimental digestion results of these respective plasmids. M: TaKaRa <t>1</t> <t>kb</t> DNA Ladder. All digested products were resolved by electrophoresis on 1% agarose gels and visualized with ethidium bromide staining. (B) Functional verification of the selection marker gene. The intermediate recombinant plasmid p15A-CmR-D90-Δ5a/AmpR-ccdB was transformed into two E. coli strains: GBred (CcdB-sensitive) and GBred- gyrA462 (CcdB-resistant). On LB agar plates containing ampicillin and chloramphenicol, only GBred- gyrA462 survived, confirming the toxicity of CcdB and functionality of the selection cassette. In the second recombination step, replacement of the AmpR-ccdB cassette by the target Δ5a/EGFP fragment yielded the final recombinant plasmid p15A-CmR-D90-Δ5a/EGFP. Upon counter-selection with CcdB, only host E. coli GBred harboring the correctly recombined plasmid survived on chloramphenicol-containing LB plates, while non-recombinant bacteria were eliminated.
    1 Kb Dna Ladder, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+dna+ladder/1+kb+DNA+Ladder/pmc13090652-136-2-1
    Average 96 stars, based on 1 article reviews
    1 kb dna ladder - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    97
    Thermo Fisher kb dna ladder
    Validation of recombinant plasmids by restriction enzyme analysis and functional selection screening. (A) Restriction enzyme digestion of intermediate recombinant plasmids was performed using XhoI, XbaI , and KpnI . The observed banding patterns were consistent with the predictions generated by SnapGene software. Lanes 1–3 show the predicted digestion patterns of p15A-CmR-D90, p15A-CmR-D90-Δ5a/AmpR-ccdB, and p15A-CmR-D90-Δ5a/EGFP. Lanes 4–5, 6–7, and 8–9 correspond to the experimental digestion results of these respective plasmids. M: TaKaRa <t>1</t> <t>kb</t> DNA Ladder. All digested products were resolved by electrophoresis on 1% agarose gels and visualized with ethidium bromide staining. (B) Functional verification of the selection marker gene. The intermediate recombinant plasmid p15A-CmR-D90-Δ5a/AmpR-ccdB was transformed into two E. coli strains: GBred (CcdB-sensitive) and GBred- gyrA462 (CcdB-resistant). On LB agar plates containing ampicillin and chloramphenicol, only GBred- gyrA462 survived, confirming the toxicity of CcdB and functionality of the selection cassette. In the second recombination step, replacement of the AmpR-ccdB cassette by the target Δ5a/EGFP fragment yielded the final recombinant plasmid p15A-CmR-D90-Δ5a/EGFP. Upon counter-selection with CcdB, only host E. coli GBred harboring the correctly recombined plasmid survived on chloramphenicol-containing LB plates, while non-recombinant bacteria were eliminated.
    Kb Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+dna+ladder/DNA+Molecular+Weight+Marker%2C+1Kb+Ladder/pm42347511-158-3-6
    Average 97 stars, based on 1 article reviews
    kb dna ladder - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    97
    Thermo Fisher generuler 1 kb plus dna ladder
    Validation of recombinant plasmids by restriction enzyme analysis and functional selection screening. (A) Restriction enzyme digestion of intermediate recombinant plasmids was performed using XhoI, XbaI , and KpnI . The observed banding patterns were consistent with the predictions generated by SnapGene software. Lanes 1–3 show the predicted digestion patterns of p15A-CmR-D90, p15A-CmR-D90-Δ5a/AmpR-ccdB, and p15A-CmR-D90-Δ5a/EGFP. Lanes 4–5, 6–7, and 8–9 correspond to the experimental digestion results of these respective plasmids. M: TaKaRa <t>1</t> <t>kb</t> DNA Ladder. All digested products were resolved by electrophoresis on 1% agarose gels and visualized with ethidium bromide staining. (B) Functional verification of the selection marker gene. The intermediate recombinant plasmid p15A-CmR-D90-Δ5a/AmpR-ccdB was transformed into two E. coli strains: GBred (CcdB-sensitive) and GBred- gyrA462 (CcdB-resistant). On LB agar plates containing ampicillin and chloramphenicol, only GBred- gyrA462 survived, confirming the toxicity of CcdB and functionality of the selection cassette. In the second recombination step, replacement of the AmpR-ccdB cassette by the target Δ5a/EGFP fragment yielded the final recombinant plasmid p15A-CmR-D90-Δ5a/EGFP. Upon counter-selection with CcdB, only host E. coli GBred harboring the correctly recombined plasmid survived on chloramphenicol-containing LB plates, while non-recombinant bacteria were eliminated.
    Generuler 1 Kb Plus Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+dna+ladder/DNA+Molecular+Weight+Marker%2C+1Kb+Ladder/pmc13255005-124-19-25
    Average 97 stars, based on 1 article reviews
    generuler 1 kb plus dna ladder - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    97
    Thermo Fisher 1 kb dna ladder
    Validation of recombinant plasmids by restriction enzyme analysis and functional selection screening. (A) Restriction enzyme digestion of intermediate recombinant plasmids was performed using XhoI, XbaI , and KpnI . The observed banding patterns were consistent with the predictions generated by SnapGene software. Lanes 1–3 show the predicted digestion patterns of p15A-CmR-D90, p15A-CmR-D90-Δ5a/AmpR-ccdB, and p15A-CmR-D90-Δ5a/EGFP. Lanes 4–5, 6–7, and 8–9 correspond to the experimental digestion results of these respective plasmids. M: TaKaRa <t>1</t> <t>kb</t> DNA Ladder. All digested products were resolved by electrophoresis on 1% agarose gels and visualized with ethidium bromide staining. (B) Functional verification of the selection marker gene. The intermediate recombinant plasmid p15A-CmR-D90-Δ5a/AmpR-ccdB was transformed into two E. coli strains: GBred (CcdB-sensitive) and GBred- gyrA462 (CcdB-resistant). On LB agar plates containing ampicillin and chloramphenicol, only GBred- gyrA462 survived, confirming the toxicity of CcdB and functionality of the selection cassette. In the second recombination step, replacement of the AmpR-ccdB cassette by the target Δ5a/EGFP fragment yielded the final recombinant plasmid p15A-CmR-D90-Δ5a/EGFP. Upon counter-selection with CcdB, only host E. coli GBred harboring the correctly recombined plasmid survived on chloramphenicol-containing LB plates, while non-recombinant bacteria were eliminated.
    1 Kb Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+dna+ladder/DNA+Molecular+Weight+Marker%2C+1Kb+Ladder/10__1007_slash_s11240___026___03498___9-90-47-50
    Average 97 stars, based on 1 article reviews
    1 kb dna ladder - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    97
    Thermo Fisher kb dna ladder molecular weight marker
    Validation of recombinant plasmids by restriction enzyme analysis and functional selection screening. (A) Restriction enzyme digestion of intermediate recombinant plasmids was performed using XhoI, XbaI , and KpnI . The observed banding patterns were consistent with the predictions generated by SnapGene software. Lanes 1–3 show the predicted digestion patterns of p15A-CmR-D90, p15A-CmR-D90-Δ5a/AmpR-ccdB, and p15A-CmR-D90-Δ5a/EGFP. Lanes 4–5, 6–7, and 8–9 correspond to the experimental digestion results of these respective plasmids. M: TaKaRa <t>1</t> <t>kb</t> DNA Ladder. All digested products were resolved by electrophoresis on 1% agarose gels and visualized with ethidium bromide staining. (B) Functional verification of the selection marker gene. The intermediate recombinant plasmid p15A-CmR-D90-Δ5a/AmpR-ccdB was transformed into two E. coli strains: GBred (CcdB-sensitive) and GBred- gyrA462 (CcdB-resistant). On LB agar plates containing ampicillin and chloramphenicol, only GBred- gyrA462 survived, confirming the toxicity of CcdB and functionality of the selection cassette. In the second recombination step, replacement of the AmpR-ccdB cassette by the target Δ5a/EGFP fragment yielded the final recombinant plasmid p15A-CmR-D90-Δ5a/EGFP. Upon counter-selection with CcdB, only host E. coli GBred harboring the correctly recombined plasmid survived on chloramphenicol-containing LB plates, while non-recombinant bacteria were eliminated.
    Kb Dna Ladder Molecular Weight Marker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+dna+ladder/1KB+DNA+LADDER/pmc13112397-147-3-9
    Average 97 stars, based on 1 article reviews
    kb dna ladder molecular weight marker - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    97
    Thermo Fisher kb pdna ladder
    Schematic representation of combined gene therapy for FD. α-Gal A: α-Galactosidase A. <t>pDNA:</t> <t>Plasmid</t> <t>DNA.</t> Gb3S: Globotriaosylceramide synthase. siRNA: Small interference RNA. FD: Fabry disease. gSRT: Genetic substrate reduction therapy. Gb3: Globotriaosylceramide.
    Kb Pdna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+dna+ladder/DNA+Molecular+Weight+Marker%2C+1Kb+Ladder/pmc13187621-65-29-33
    Average 97 stars, based on 1 article reviews
    kb pdna ladder - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    97
    Thermo Fisher generuler 1 kb dna ladder
    Schematic representation of combined gene therapy for FD. α-Gal A: α-Galactosidase A. <t>pDNA:</t> <t>Plasmid</t> <t>DNA.</t> Gb3S: Globotriaosylceramide synthase. siRNA: Small interference RNA. FD: Fabry disease. gSRT: Genetic substrate reduction therapy. Gb3: Globotriaosylceramide.
    Generuler 1 Kb Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+dna+ladder/DNA+Molecular+Weight+Marker%2C+1Kb+Ladder/pmc13199811-79-17-22
    Average 97 stars, based on 1 article reviews
    generuler 1 kb dna ladder - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    97
    Thermo Fisher kb molecular weight marker
    Schematic representation of combined gene therapy for FD. α-Gal A: α-Galactosidase A. <t>pDNA:</t> <t>Plasmid</t> <t>DNA.</t> Gb3S: Globotriaosylceramide synthase. siRNA: Small interference RNA. FD: Fabry disease. gSRT: Genetic substrate reduction therapy. Gb3: Globotriaosylceramide.
    Kb Molecular Weight Marker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+dna+ladder/DNA+Molecular+Weight+Marker%2C+1Kb+Ladder/10__3390_slash_phycology6020060-73-12-16
    Average 97 stars, based on 1 article reviews
    kb molecular weight marker - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    Image Search Results


    Validation of recombinant plasmids by restriction enzyme analysis and functional selection screening. (A) Restriction enzyme digestion of intermediate recombinant plasmids was performed using XhoI, XbaI , and KpnI . The observed banding patterns were consistent with the predictions generated by SnapGene software. Lanes 1–3 show the predicted digestion patterns of p15A-CmR-D90, p15A-CmR-D90-Δ5a/AmpR-ccdB, and p15A-CmR-D90-Δ5a/EGFP. Lanes 4–5, 6–7, and 8–9 correspond to the experimental digestion results of these respective plasmids. M: TaKaRa 1 kb DNA Ladder. All digested products were resolved by electrophoresis on 1% agarose gels and visualized with ethidium bromide staining. (B) Functional verification of the selection marker gene. The intermediate recombinant plasmid p15A-CmR-D90-Δ5a/AmpR-ccdB was transformed into two E. coli strains: GBred (CcdB-sensitive) and GBred- gyrA462 (CcdB-resistant). On LB agar plates containing ampicillin and chloramphenicol, only GBred- gyrA462 survived, confirming the toxicity of CcdB and functionality of the selection cassette. In the second recombination step, replacement of the AmpR-ccdB cassette by the target Δ5a/EGFP fragment yielded the final recombinant plasmid p15A-CmR-D90-Δ5a/EGFP. Upon counter-selection with CcdB, only host E. coli GBred harboring the correctly recombined plasmid survived on chloramphenicol-containing LB plates, while non-recombinant bacteria were eliminated.

    Journal: Poultry Science

    Article Title: Construction and modification of a low-copy plasmid-based infectious clone for GI-19 genotype IBV via Red/ET recombineering: A simplified and efficient reverse genetics system for co ronavirus

    doi: 10.1016/j.psj.2026.106881

    Figure Lengend Snippet: Validation of recombinant plasmids by restriction enzyme analysis and functional selection screening. (A) Restriction enzyme digestion of intermediate recombinant plasmids was performed using XhoI, XbaI , and KpnI . The observed banding patterns were consistent with the predictions generated by SnapGene software. Lanes 1–3 show the predicted digestion patterns of p15A-CmR-D90, p15A-CmR-D90-Δ5a/AmpR-ccdB, and p15A-CmR-D90-Δ5a/EGFP. Lanes 4–5, 6–7, and 8–9 correspond to the experimental digestion results of these respective plasmids. M: TaKaRa 1 kb DNA Ladder. All digested products were resolved by electrophoresis on 1% agarose gels and visualized with ethidium bromide staining. (B) Functional verification of the selection marker gene. The intermediate recombinant plasmid p15A-CmR-D90-Δ5a/AmpR-ccdB was transformed into two E. coli strains: GBred (CcdB-sensitive) and GBred- gyrA462 (CcdB-resistant). On LB agar plates containing ampicillin and chloramphenicol, only GBred- gyrA462 survived, confirming the toxicity of CcdB and functionality of the selection cassette. In the second recombination step, replacement of the AmpR-ccdB cassette by the target Δ5a/EGFP fragment yielded the final recombinant plasmid p15A-CmR-D90-Δ5a/EGFP. Upon counter-selection with CcdB, only host E. coli GBred harboring the correctly recombined plasmid survived on chloramphenicol-containing LB plates, while non-recombinant bacteria were eliminated.

    Article Snippet: M: TaKaRa 1 kb DNA Ladder.

    Techniques: Biomarker Discovery, Recombinant, Functional Assay, Selection, Generated, Software, Electrophoresis, Staining, Marker, Plasmid Preparation, Transformation Assay, Bacteria

    Schematic representation of combined gene therapy for FD. α-Gal A: α-Galactosidase A. pDNA: Plasmid DNA. Gb3S: Globotriaosylceramide synthase. siRNA: Small interference RNA. FD: Fabry disease. gSRT: Genetic substrate reduction therapy. Gb3: Globotriaosylceramide.

    Journal: International Journal of Pharmaceutics: X

    Article Title: Nanomedicines for DNA and interference RNA co-delivery: Combined gene therapy for Fabry disease

    doi: 10.1016/j.ijpx.2026.100555

    Figure Lengend Snippet: Schematic representation of combined gene therapy for FD. α-Gal A: α-Galactosidase A. pDNA: Plasmid DNA. Gb3S: Globotriaosylceramide synthase. siRNA: Small interference RNA. FD: Fabry disease. gSRT: Genetic substrate reduction therapy. Gb3: Globotriaosylceramide.

    Article Snippet: Agarose, deoxyribonuclease I (DNAse I) and sodium dodecyl sulfate (SDS) were purchased from Sigma-Aldrich, GelRedTM from Biotium (Fremont, CA, USA) and GeneRuler Ultra Low Range DNA Ladder and 1 Kb pDNA ladder from Gibco (Thermo Fisher Scientific, MA, USA).

    Techniques: Plasmid Preparation

    Binding, protection and release capacity of co-delivery vectors with SLNs containing DOTAP or DOTAP/MC3. In all gels, lane 1 corresponds to 1 Kb pDNA ladder. Lane 2 to naked pCMV6-AC-αGLA, lane 3 to naked pCMV6-AC-αGLA + SDS, lane 4 to naked siRNA, lane 5 to siRNA + SDS, lane 6 to siRNA-GN + SDS, lane 11 to naked pR-M10-αGal A and lane 12 to Naked siRNA + DNase + SDS. ( A ) DxP3GNSLNEE co-delivery vectors: (7) DxP3GN5-ET. (8) DxP3GN2.5-ET. (9) DxP3GN5-EMC3. (10) DxP3GN2.5-EMC3. (13) DxP3GN5-ET vector + DNase + SDS. (14) DxP3GN2.5-ET vector + DNase + SDS. (15) DxP3GN5-EMC3 + DNase + SDS. (16) DxP3GN2.5-EMC3 + DNase + SDS. (17) DxP3GN5-ET + SDS. (18) DxP3GN2.5-ET + SDS. (19) DxP3GN5-EMC3 + SDS. (20) DxP3GN2.5-EMC3 + SDS. (B) HAP3GNSLNEE co-delivery vectors: (7) HAP3GN5-ET. (8). HAP3GN2.5-ET. (9) HAP3GN5-EMC3. (10) HAP3GN2.5-EMC3. (13) HAP3GN5-ET + DNase + SDS. (14) HAP3GN2.5-ET + DNase + SDS. (15) HAP3GN5-EMC3 + DNase + SDS. (16) HAP3GN2.5-EMC3 + DNase + SDS. (17) HAP3GN5-ET + SDS. (18) HAP3GN2.5-ET + SDS. (19) HAP3GN5-EMC3 + SDS. (20) HAP3GN2.5-EMC3 + SDS. ( C ) DxP2GNSLNEE co-delivery vectors: (7) DxP2GN5-ET. (8). DxP2GN2.5-ET. (9) DxP2GN5-EMC3. (10) DxP2GN2.5-EMC3. (13) DxP3GN5-ET + DNase + SDS. (14) DxP3GN2.5-ET + DNase + SDS. (15) DxP2GN5-EMC3 + DNase + SDS. (16) DxP2GN2.5-EMC3 + DNase + SDS. (17) DxP2GN5-ET + SDS. (18) DxP2GN2.5-ET + SDS. (19) DxP2GN5-EMC3 + SDS. (20) DxP2GN2.5-EMC3 + SDS. ( D ) HAP2GNSLEE co-delivery vectors: (7) HAP2GN5-ET. (8). HAP2GN2.5-ET. (9) HAP2GN5-EMC3. (10) HAP2GN2.5-EMC3. (13) HAP2GN5-ET + DNase + SDS. (14) HAP2GN2.5-ET + DNase + SDS. (15) HAP2GN5-EMC3 + DNase + SDS. (16) HAP2GN2.5-EMC3 + DNase + SDS. (17) HAP2GN5-ET + SDS. (18) HAP2GN2.5-ET + SDS. (19) HAP2GN5-EMC3 + SDS. (20) HAP2GN2.5-EMC3 + SDS.

    Journal: International Journal of Pharmaceutics: X

    Article Title: Nanomedicines for DNA and interference RNA co-delivery: Combined gene therapy for Fabry disease

    doi: 10.1016/j.ijpx.2026.100555

    Figure Lengend Snippet: Binding, protection and release capacity of co-delivery vectors with SLNs containing DOTAP or DOTAP/MC3. In all gels, lane 1 corresponds to 1 Kb pDNA ladder. Lane 2 to naked pCMV6-AC-αGLA, lane 3 to naked pCMV6-AC-αGLA + SDS, lane 4 to naked siRNA, lane 5 to siRNA + SDS, lane 6 to siRNA-GN + SDS, lane 11 to naked pR-M10-αGal A and lane 12 to Naked siRNA + DNase + SDS. ( A ) DxP3GNSLNEE co-delivery vectors: (7) DxP3GN5-ET. (8) DxP3GN2.5-ET. (9) DxP3GN5-EMC3. (10) DxP3GN2.5-EMC3. (13) DxP3GN5-ET vector + DNase + SDS. (14) DxP3GN2.5-ET vector + DNase + SDS. (15) DxP3GN5-EMC3 + DNase + SDS. (16) DxP3GN2.5-EMC3 + DNase + SDS. (17) DxP3GN5-ET + SDS. (18) DxP3GN2.5-ET + SDS. (19) DxP3GN5-EMC3 + SDS. (20) DxP3GN2.5-EMC3 + SDS. (B) HAP3GNSLNEE co-delivery vectors: (7) HAP3GN5-ET. (8). HAP3GN2.5-ET. (9) HAP3GN5-EMC3. (10) HAP3GN2.5-EMC3. (13) HAP3GN5-ET + DNase + SDS. (14) HAP3GN2.5-ET + DNase + SDS. (15) HAP3GN5-EMC3 + DNase + SDS. (16) HAP3GN2.5-EMC3 + DNase + SDS. (17) HAP3GN5-ET + SDS. (18) HAP3GN2.5-ET + SDS. (19) HAP3GN5-EMC3 + SDS. (20) HAP3GN2.5-EMC3 + SDS. ( C ) DxP2GNSLNEE co-delivery vectors: (7) DxP2GN5-ET. (8). DxP2GN2.5-ET. (9) DxP2GN5-EMC3. (10) DxP2GN2.5-EMC3. (13) DxP3GN5-ET + DNase + SDS. (14) DxP3GN2.5-ET + DNase + SDS. (15) DxP2GN5-EMC3 + DNase + SDS. (16) DxP2GN2.5-EMC3 + DNase + SDS. (17) DxP2GN5-ET + SDS. (18) DxP2GN2.5-ET + SDS. (19) DxP2GN5-EMC3 + SDS. (20) DxP2GN2.5-EMC3 + SDS. ( D ) HAP2GNSLEE co-delivery vectors: (7) HAP2GN5-ET. (8). HAP2GN2.5-ET. (9) HAP2GN5-EMC3. (10) HAP2GN2.5-EMC3. (13) HAP2GN5-ET + DNase + SDS. (14) HAP2GN2.5-ET + DNase + SDS. (15) HAP2GN5-EMC3 + DNase + SDS. (16) HAP2GN2.5-EMC3 + DNase + SDS. (17) HAP2GN5-ET + SDS. (18) HAP2GN2.5-ET + SDS. (19) HAP2GN5-EMC3 + SDS. (20) HAP2GN2.5-EMC3 + SDS.

    Article Snippet: Agarose, deoxyribonuclease I (DNAse I) and sodium dodecyl sulfate (SDS) were purchased from Sigma-Aldrich, GelRedTM from Biotium (Fremont, CA, USA) and GeneRuler Ultra Low Range DNA Ladder and 1 Kb pDNA ladder from Gibco (Thermo Fisher Scientific, MA, USA).

    Techniques: Binding Assay, Plasmid Preparation